Protein Phosphorylation : A Practical Approach (2nd ed)

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells.

This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it.

It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates.

Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases.

Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.